| 质粒名称: | eGFPC1 FKBP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 67529 |
| 存储实验室: | Stephen Tait |
|
载体骨架基本信息 |
|
| 载体名称: | eGFPC1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4500 bp |
| 完整质粒大小: | 5043 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FKBP |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 321 bp |
| 基因突变: | F36V |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | eGFP（插入端N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI (未破坏) |
| 3' 克隆位点: | BamH1(未破坏) |
| 5' 测序引物 | CMV |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | eGFPC1 FKBP was a gift from Stephen Tait (Addgene plasmid # 67529 ; http://n2t.net/addgene:67529 ; RRID:Addgene_67529) |
| 参考文献部分: | Limited mitochondrial permeabilization causes DNA damage and genomic instability in the absence of cell death. Ichim G, Lopez J, Ahmed SU, Muthalagu N, Giampazolias E, Delgado ME, Haller M, Riley JS, Mason SM, Athineos D, Parsons MJ, van de Kooij B, Bouchier-Hayes L, Chalmers AJ, Rooswinkel RW, Oberst A, Blyth K, Rehm M, Murphy DJ, Tait SW. Mol Cell. 2015 Mar 5;57(5):860-72. doi: 10.1016/j.molcel.2015.01.018. Epub 2015 Feb 19. 10.1016/j.molcel.2015.01.018 PubMed 25702873 |
买家导航
eGFPC1FKBP载体基本信息
eGFPC1FKBP载体图谱质粒图谱和多克隆位点信息
eGFPC1FKBP载体简介
eGFPC1FKBP载体序列
LOCUS eGFPC1 FKBP 5043 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses GFP fused FKBP.
ACCESSION .
VERSION .
KEYWORDS eGFPC1 FKBP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5043)
AUTHORS Ichim G, Lopez J, Ahmed SU, Muthalagu N, Giampazolias E, Delgado ME,
Haller M, Riley JS, Mason SM, Athineos D, Parsons MJ, van de Kooij
B, Bouchier-Hayes L, Chalmers AJ, Rooswinkel RW, Oberst A, Blyth K,
Rehm M, Murphy DJ, Tait SW
TITLE Limited mitochondrial permeabilization causes DNA damage and genomic
instability in the absence of cell death.
JOURNAL Mol Cell. 2015 Mar 5;57(5):860-72. doi:
10.1016/j.molcel.2015.01.018. Epub 2015 Feb 19.
PUBMED 25702873
REFERENCE 2 (bases 1 to 5043)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5043
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 607..616
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 613..1329
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(658..679)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(919..938)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1266..1287
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1831..1952
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1868..1887)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1922..1941
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1959..2414)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2096..2117)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2308..2327
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2441..2545
/gene="bla"
/label=AmpR promoter
promoter 2547..2904
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2570..2590)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2755..2890
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2817..2836
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2939..3733
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2993..3012)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3603..3622
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3921..3940)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3965..4012
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4341..4929
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4830..4849
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctggagg tggcggggga
1381 gtgcaggtgg aaactatctc cccaggagac gggcgcacct tccccaagcg cggccagacc
1441 tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aagttgattc ctcccgggac
1501 agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa
1561 ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc
1621 tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg
1681 gagctgctga agctggagtg aggatccacc ggatctagat aactgatcat aatcagccat
1741 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
1801 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
1861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
1921 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
1981 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
2041 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
2101 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
2161 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
2221 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
2281 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
2341 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
2401 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
2461 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
2521 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
2581 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
2641 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
2701 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
2761 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
2821 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
2881 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
2941 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
3001 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
3061 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
3121 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
3181 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
3241 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
3301 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
3361 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
3421 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
3481 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
3541 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
3601 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
3661 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
3721 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
3781 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
3841 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
3901 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
3961 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
4021 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
4081 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
4141 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
4201 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
4261 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
4321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
4381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
4441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
4501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
4561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
4621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
4681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
4741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
4801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
4861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
4921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
4981 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
5041 cat
//
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